Immediately after peptide bond formation, which site contains the peptidyl-tRNA?

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Multiple Choice

Immediately after peptide bond formation, which site contains the peptidyl-tRNA?

Explanation:
In translation, the growing polypeptide is transferred from the tRNA in the P site to the tRNA that has just entered in the A site during the peptidyl transferase step. Because the bond formation happens between the A-site tRNA’s amino group and the nascent chain on the P-site tRNA, the peptidyl-tRNA ends up in the A site immediately after the bond is formed. Only after this step does translocation move that peptidyl-tRNA to the P site (and the deacylated tRNA to the E site). The A site is, at that moment, the location of the peptidyl-tRNA.

In translation, the growing polypeptide is transferred from the tRNA in the P site to the tRNA that has just entered in the A site during the peptidyl transferase step. Because the bond formation happens between the A-site tRNA’s amino group and the nascent chain on the P-site tRNA, the peptidyl-tRNA ends up in the A site immediately after the bond is formed. Only after this step does translocation move that peptidyl-tRNA to the P site (and the deacylated tRNA to the E site). The A site is, at that moment, the location of the peptidyl-tRNA.

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